Gambling disorder involves recurring gambling behaviour that is problematic. Gambling disorder is linked to issues such as substance use disorders, psychological distress, and suicide. Lesbian, gay, bisexual, transgender, queer, intersexual, asexual, two-spirit (LGBTQIA2S+) populations are more likely to have some of these issues. This scoping review summarized the research on gambling behaviours and gambling disorder among LGBTQIA2S+ populations. The authors identified six research studies that were published in English. There was a considerable mix of methods and results across all six of the studies. Each of the studies examined problem gambling using different tools. All six studies compared people who identified as non-heterosexual and those who identified as heterosexual. The authors of this review concluded that the current research does not show whether gambling disorder is more common among LGBTQIA2S+ populations.